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KMID : 0380019930080050465
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Korean Journal of Biotechnology and Bioengineering
1993 Volume.8 No. 5 p.465 ~ p.471
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The Cultivation of Anchorage-Dependent Animal Cell, Vero-6, on Marcroporous Collagen Microcarrier
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Abstract
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The comparison of the capabilities of cell growth of four different kinds of commercially available microcarriers was carried out by culturing anchorage-dependent animal cells, Vero-6, in a spinner flask. Using 3 g/l of Cytodex ;the maximum final cell density was about 1.4 X 106 cells/ml and increased up to 2.0 x 106 cells/ml by increasing microcarrier concentration up to 5 g/l. The macroporous collagen microcarriers, VX-100, Informatrix, and Cultispher-G showed the final cell concentration of 4. 4 x 106 cells/ml, 2.1 x 106 cells/ml, and 3.2 x 106 cells/ml, respectively at the microcarrier concentration of 5 g/1. According to this result, VX-100 showed better cell growth than Informatrix and cultispher-G and also showed about 2 fold increase in final cell density comparing to Cytodex 3 solid bead. When the intermittent bead-to-bead transfer technique was introduced in the culture using Cytodex 3 bead and Cultispher-G, the result was very successful and the cells grew out very well. The recovered cells by dissolving collagen microcarrier using collagenase enzyme were mostly viable and grew out very well on the surface of the fresh microcarriers.
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